Purification and identification of bacterial cellulase activity of Bacillus subtilis W48 for biofuel production
Purification and identification of bacterial cellulase activity of Bacillus subtilis W48 for biofuel production
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2014-07
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School of Biotechnology, Assumption University of Thailand
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eng
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application/pdf
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4 pages
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International Conference on Sustainable Global Agricultural and Food Security
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Abstract
Lignocellulosic biomass is a renewable, inexpensive, and abundant resource with high potential for biofuel production to implement the sustainable energy worldwide. An
important key of biofuel
production
is the hydrolysis of
lignocellulosic biomass to
fermentable sugars. Screening for a novel cellulase, as a biocatalyst, is challenge for
development of
biofuel production to
be the
economic
and environmental friendly
process. Natural environment is an
important
source to
find an efficient cellulase
producing bacteria. Previously, Bacillus subtilis strain W48 was screened and isolated
from
rainforest
park at Assumption
University,
Bangkok,
Thailand. In this study, we
focused on the
purification
and characterization
of cellulase enzyme
produced
by B.
subtilis strain W48. The crude cellulase enzyme was collected by culture grown at 45°C in carboxy-methyl-cellulose (CMC) media. Then, it is concentrated by ammonium sulfate precipitation and fractionated by using size exclusion chromatography using sephacryl S-
100
HR column. In this study, the fractions that has highest CMCase activity (0.017
mg/ml), as endoglucanase, and FPase activity (0.013 mg/ml), exoglucanase, are fraction no. 13 and 11, respectively.