Browsing by Author "Viyada Kunathigan"
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    Detection of Orcinol-O-methyl transferase enzyme encoding gene in East Asian rose Rosa multiflora
    ( 2014) Viyada Kunathigan
    3,5-dimethoxytoluene (DMT) is a key component of “tea scent” in Chinese rose (Rosa chinesis) and a hybrid of Chinese rose (Rosa X hybrida). DMT biosynthesis is catalysed by enzymes Orcinol O-methyltransferase (OOMT) 1 and 2 (Rosa chinesis and Rosa X hybrida) and OOMT like (Rosa chinesis Xing-xing-hei). The East Asian rose Rosa multiflora may also contain OOMT gene. The presence of OOMT gene in this rose could be a potential useful resource for Asian fragrance industry. This project aimed to detect the OOMT encoding gene in this rose variety. Rosa multiflora var. carnea and Rosa multiflora platyphylla were chosen as plant materials as they are abundant in South East Asian area. DNA extraction and primer design were achieved to amplify OOMT gene. The designed primers were expected to amplify all OOMT1, OOMT2, and OOMT like genes. With DNA templates and a pair of primers, PCR was conducted and showed the presence of OOMT gene in Rosa multiflora var. carnea (product size around 1200bp) but not in Rosa multiflora platyphylla. The 1200bp product size was close to the expected size of OOMT like gene’s PCR product (1211bp). A restriction enzyme digestion analysis was done and the results supported that the OOMT gene in Rosa multiflora var. carnea was OOMT like gene.
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    Screening and primary identification of lipid degradation microorganism from local bioextracts
    ( 2014) Viyada Kunathigan
    Bioextract is a solution of fermented organic matters which mainly are agricultural leftover containing living microorganisms. Studies have shown that bioextract may be applied to agriculture, livestock, gardening and landscaping, composting, and bioremediation. Latterly, bioextract was introduced for wastewater treatment, due to bioextract characteristic and environmentally friendly. From previous studies (Nitsuwat et al., 2013), addition of bioextract was capable to reduce total solid, grease and oil in domestic wastewater. The grease and oil component in wastewater can create problem in sewage system and hard to lose from the wastewater. The aim of this research is to find and identify microorganism contained inside the bioextract that can degrade grease and oil. In the future increasing of these microorganisms in bioextract may improve the ability of bioextract for pretreating domestic wastewater containing grease and oil. In this research, lipid degradation microorganisms were isolated from the local bioextract sample. The bioextract biodivesity was found contained total aerobic bacteria 1.07x104 CFU.ml-1, actinomycetes 4.53x104 CFU.ml-1, yeast 2.67x104 CFU.ml-1, lactic acid bacteria 3.04 x 103 CFU.ml-1, and mold 1.98x103 CFU.ml-1. The screening methods used media added with tributyrin. Colonies that created clear zone were isolated and tested using tributyrin and vegetable oil. Total of 55 microorganisms were screened for their ability to degrade tributyrin. Ten strains were further selected from the second screening using vegetable oil. Then these ten isolates were primarily identified using morphological and biochemical characteristics. Majority of the selected isolates were in family Bacillaceae.
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    The study of the biodiversity in local bio-fermented solution and the treatment of community wastewater at laboratory scale: wastewater from restaurants
    (Kasetsart University, 2013-10) Viyada Kunathigan
    Bio-fermented solution is a solution composed of a diverse variety of microbes coexisting together, aiding the metabolism of each other. Nowadays,bio-fermented solution is widely used for many purposes and is one of the popular usages for wastewater treatment. In this experiment, the biodiversity of the bio-fermented solution was determined by selective and non-selective enrichment media. For the treatment of restaurant wastewater (collected from local department store food court and steak restaurant), 0 (control), 0.25, 0.5 and 1 mL of bio-fermented solution were inoculated per liter of wastewater. In the treatment procedure, light (with light and without light), time (24 hours and 48 hours) and oxygen (with oxygen and without oxygen) were varied. After treatment, the wastewater sample’s chemical and microbiological properties were tested. The chemical properties include total solid (TS), Biochemical oxygen demand (BOD), total suspended solid (TSS), total dissolved solid (TDS), pH and grease and oilcontent. The microbiological properties were measured by MPN method and total plate count method. The bio- fermented solution biodiversitieswere contained with Bacillus spp. 3.48×104 CFU.mL-1, mold 3.63×103 CFU.mL-1, lactic acid bacteria 4.35×104 CFU.mL-1, actinomycetes 1.27×105 CFU.mL-1 and yeast 1.35×105 CFU.mL-1. When using bio- fermented solution to treat restaurant wastewater, there were significant reduction of TS and grease and oil at 53.07% and 69.89% respectively. The best condition for restaurant wastewater treatment was 0.25 mL of bio-fermented solution per liter of wastewater without oxygen and light for 48 hours. However, the quality of treated wastewater was still above the standard required. Therefore, further experiment will be needed to improve the quality of water before discarding.