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Browsing by Subject "Antioxidant"

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  • Item
    Aloe vera gel: effect of mangosteen extract in the presence of vitamin C on antioxidant properties
    (Bangkok : Assumption University, 2019) Phathit Ruengwech
  • Item
    Antibacterial activity, antioxidant activity and chemical profiling of centella asiatica under different extraction solvents
    (Bangkok : Assumption University, 2014) Supawan Rattanakom ; Patchanee Yasurin
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    The Development of Centella asiatica Extract-Loaded BSA Nanoparticles Production to Improve Bioavailability
    (Assumption University, ) Patchanee Yasurin
    Centella asiatica cBao-bog) is used as a traditional drug widely in Asia. C. asiatica crude extracts showed excellent potential in-vitro but less or no in-vivo activity due to their poor lipid solubility or improper molecular size or both, resulting in poor absorption, poor dosing and poor bioavailability. The Bovine serum albumin cBSA) can attract macromolecular and carry wide variety of molecule. So this research was aimed to develop C. asiatica extract-loaded BSA nanoparticles cCBNP) to improve bioavailability. CBNP was prepared by the desolvation method using three different ratio C. asiatica crude chloroform extracts: BSA cl:2, 1:3, and 1:4). The well agar diffusion method was used for evaluating antibacterial activity of CBNP with different concentration (100, 200, and 300 µg!Illl) against five food borne pathogens cEscherichia coli ATCC25822, Salmonella enterica Typhimurium U302 cDT104b), S. enterica Enteritidis chuman), S. enterica 4,5,12:i:- chumall) US clone, and Bacillus cereus). The results showed that the antibacterial activity of CBNP did not show significant different on three different ratio and concentration in all food borne pathogens except S. enterica Enteritidis chuman) and B. cereus cP < 0.05). The highest antibacterial active of CBNP was 1.07±0.46 cm against S. enterica Enteritidis (human) on ratio 1 :4, 200 µg!Illl. The antibacterial activity of CBNP gave almost 2 times highe than free crude C. asiatica chloroform extracts. The modified Folin-Ciocalteu method, Ferric reducing antioxidant potential assay and DPPH assay were used for evaluating antioxidant activity. The highest antioxidant activity represented by the amount of phenolic content of CBNP was 14.59±6.74 µgGAEIIllg using ratio 1:2. The amount of phenolic content of CBNP did not show significant different between ratio 1 :2 and others cP < 0.05). For Ferric reducing antioxidant potential assay and DPPH assay, crude chloroform extract showed significantly higher activity than CBNP were 1.00 ± 0.15 mmol Fe2 + 1Illg and 29.44 ± 8.20 % DPPH radical scavenging respectively cP < 0.05). The entrapment efficiency and loading efficiency of CBNP showed highest value on ratio 1 :4 which were 96.94 ± 1.48% and 42.05 ± 5.68% respectively. It's also showed highest in release kinetic In Vitro approximately 12% during the whole period of 6 hours in both artificial gastric and intestinal juice. C. asiatica crude chloroform extract have higher solubility in water than CBNP but CBNP have higher stability in releasing crude chloroform extract. The results indicated that CBNP showed the promising to increase bioavailability of C. asiatica.
  • Item
    Development of herbal Khlu tea, chemical analysis and sensory analysis
    (Bangkok : Assumption University, 2018) Surasee Promchun
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    The development of Phyllanthus emblica extract-loaded BSA nanoparticles production to improve bioavailability
    (Bangkok : Assumption University, 2016) Win, New New
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    Microencapsulation of bamboo leaves extract from Pai Tong (Dendocalamus asper J.H. Schuttes) and Pai Ruak (Thyrsostachys siamensis gamble) and their antioxidant properties
    (Bangkok : Assumption University, 2020) Roungdao Klinjapo ; Wunwisa Krasaekoopt
  • Item
    The Overview: the antimicrobial, antioxidant activities and chemical profile of Centella asiatica
    (School of Biotechnology, Assumption University of Thailand, 2014-07) Patchanee Yasurin
    According to the draft of Thai herbals development strategic plan; Thailand Champion Herbal Products: TCHP by the department for development of Thai transitional and alternative medicine, Ministry of Public Health, Centella asiatica (Bao-bog, Gotu Kola) is one of the five herbs in champion herbal products. To be able to produce quality herbal products, scientific proven data is needed to increase the confidence and acceptance of consumers, not only local wisdom. The variation in herbal form (fresh and dried), solvent types, and extraction ratio and extraction times could affect the antimicrobial activities, antioxidant activities and chemical profile. Thus that biological active compound compositions, antimicrobial activity, and antioxidant activity profiles would be the important stepping stone for development of medicine industry, food industry, cosmetics industry.
  • Item
    Review: antibacterial, antioxidant and chemical profile of centella asiatica
    ( 2014) Supawan Rattanakom ; Patchanee Yasurin
    Plants have been used since ancient times whether as foods/seasoning or medicinal purposes. The used of plants for medicinal purposes can be found almost every parts of the world; from Africa to Europe. Nowadays, there are increasing trends of using natural products. From the discovery that effective life span of any antibiotic is limited. Scientists try to discover alternatives. One of the sources is from plants. As they have an almost limitless ability to synthesize aromatic substances especially plant secondary metabolites. Most of plant secondary metabolites serve as plant defense mechanisms against predation by microorganisms, insects, and herbivores. Thus this is where the hypothesis of using plants as alternative antimicrobial agents. Not only that, plant also contains phenolic compounds that can be served as natural antioxidants. Interestingly herbs are considered to be one of the alternatives consumers choose to use. In Thailand, Centella asiatica can be found in local market and locally called Buabok. C. asiatica is famous in Ayurvedic medicine for the treatment of leprosy, insanity, asthma, ulcers, eczema, skin tuberculosis, wounds, stomach aches, arthritis, varicose veins and high blood pressure. By that, gathered information of antibacterial, antioxidant and chemical profile of C. asiatica could serve as stepping stone.

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