Browsing by Subject "Antioxidant activity"
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ItemAntioxidant activity of Allium tuberosum Rottl. ex Spreng under different extraction methods( 2015-11) Patchanee Yasurin ; Napatsorn Lawthienchai ; Aussama SoontrunnarudrungsriThe Bang-Phae organic vegetable grower community enterprise group, Ratchaburi province is one of the big Chinese chives (Allium tuberosum Rottl. ex Spreng) grower in Thailand. However, some Chinese chives did not meet standard and were sorted out. The Chinese chives essential oil, which contains many new and known bioactive compounds, is valued product from sorted out Chinese chives. Therefore, this experiment was aimed to study the antioxidant activity of Chinese chive extracts using 17 different extraction conditions (Steam distillation for 1.0, 2.0, 2.5, 3.0 hour; Ohmic pretreatment with steam distillation for 1.0, 2.0, 2.5, 3.0 hour; 95% ethanol, hexane, oil extract using dry, fresh, freeze Chinese chives). The ferric reducing antioxidant potential assay was used to evaluate antioxidant activity of Chinese chive extracts. The results showed that the highest antioxidant activity was 1.756 ± 0.008 µmol Fe2+/ mg sample of freeze Chinese chives extracted with hexane. The antioxidant activity was vary from 0.098 ± 0.005 - 1.756 ± 0.008 µmol Fe2+/ mg sample depend on the extraction condition.
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ItemChemical profiling of centella asiatica under different extraction solvents and its antibacterial activity, antioxidant activity( 2015) Patchanee YasurinCentella asiatica (L) urban, synonym Hydrocotyle asiatica, is found almost all over the world. This plant is famous in Ayurvedic medicine and used in the management of central nervous system, skin and gastrointestinal disorder. Thus this research had been done to evaluate the effect of solvent extraction (Ethanol, Chloroform and Hexane) of C. asiatica on chemical profile, antioxidant activity and antibacterial activity against some foodborne pathogens. The result showed that all solvents (ethanol, chloroform and hexane) used in extraction showed antibacterial activity against Salmonella enterica Typhimurium U302, S. enterica Enteritidis, S. enterica 4,5,12:I human (US clone), Bacillus cereus and B. subtilis at 50mg/ml concentration. In antioxidant part, ethanolic extract gave highest phenolic content and FRAP value. The results also showed that different extraction solvent gave different chemical profile. Hexane extract C. asiatica showed lowest in both antibacterial and antioxidant activity. Ethanolic and chloroform extract of C. asiatica showed promising potential in both antibacterial and antioxidant activity.
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ItemEffect of solvent extraction of Centella asiatica on antioxidant activity and antibacterial activity against Salmonella enterica EnteritidisNowadays, there are increasing trends of using natural products. Interestingly herbs are considered to be one of the alternatives consumers choose to use. In Thailand, Centella asiatica can be found in local market and locally called Buabok. C. asiatica is famous in Ayurvedic medicine for the treatment of leprosy, insanity, asthma, ulcers, eczema, skin tuberculosis, wounds, stomach aches, arthritis, varicose veins and high blood pressure (Ariffin et al, 2011 and Hakono et al, 1999). Due to the benefits of C. asiatica and its easily accessible, this studied had been done to evaluate the effect of solvent extraction of C. asiatica on antibacterial activity against Salmonella enterica Enteritidis and found out that ethanol and chloroform showed highest antibacterial activity against S. Enteritidis (9.33 ± 0.5774 and 9.50 ± 0.5000 mm., respectively) while hexane extract showed lowest activity (6.67 ± 0.1443 mm.) The minimum inhibitory concentrations (MICs), using a broth dilution method, were found to be 8 mg/ml for ethanol and chloroform. While hexane extract was found to be 32 mg/ml. The minimum bactericidal concentrations (MBCs) were greater than 32 mg/ml. Similar results were found in the antioxidant activity of C. asiatica referring to the amount of phenolic content. Total phenolic contents using Folin-Ciocalteu method, found out that ethanol extract contains highest phenolic content followed by chloroform with slightly lower in phenolic content and the lowest phenolic content was hexane extract (23.8020 ± 0.5241, 22.1718 ± 0.1403 and 7.9612 ± 1.6350 µg GAE/mg, respectively). Ferric reducing antioxidant potential value of ethanolic extract was greater than those of chloroform and hexane extract (6.4008 ± 0.0393, 3.4779 ± 0.6744 and 1.7693 ± 0.1279 mmol Fe2+/mg, respectively). According to the results, different extraction solvents affected the amount of total phenolic compounds and FRAP value. The difference in the antibacterial activity was found when using different extraction solvents.
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