Browsing by Subject "Flavonoid"
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ItemDevelopment of herbal Khlu tea, chemical analysis and sensory analysis(Bangkok : Assumption University, 2018) Surasee Promchun
ItemNumber of hydroxyl groups on the B-ring of flavonoids affects their antioxidant activity and interaction with phorbol ester binding site of PKCδ C1B domain: in Vitro and in silico studiesAlthough ﬂavonoids have been reported for their beneﬁts and nutraceutical potential use, the importance of their structure on their beneﬁcial eﬀects, especially on signal transduction mechanisms, has not been well clariﬁed. In this study, three ﬂavonoids, pinocembrin, naringenin, and eriodictyol, were chosen to determine the eﬀect of hydroxyl groups on the B-ring of ﬂavonoid structure on their antioxidant activity. In vitro assays, including DPPH scavenging activity, ROS quantiﬁcation by ﬂow cytometer, and proteins immunoblotting, and in silico analysis by molecular docking between the ﬂavonoids and C1B domain of PKCδ phorbol ester binding site were both used to complete this study. Eriodictyol (10 μM), containing two hydroxyl groups on the B-ring, exhibited signiﬁcantly higher (p < 0.05) antioxidant activity than pinocembrin and naringenin. The IC50 values of eriodictyol, naringenin, and pinocembrin were 17.4 ± 0.40, 30.2 ± 0.61, and 44.9 ± 0.57 μM, respectively. In addition, eriodictyol at 10 μM remarkably inhibited the phosphorylation of PKCδ at 63.4% compared with PMA-activated RAW264.7, whereas pinocembrin and naringenin performed inhibition activity at 76.8 and 72.6%, respectively. According to the molecular docking analysis, pinocembrin, naringenin, and eriodictyol showed -CDOCKER_energy values of 15.22, 16.95, and 21.49, respectively, reﬂecting that eriodictyol could bind with the binding site better than the other two ﬂavonoids. Interestingly, eriodictyol had a remarkably diﬀerent pose to bind with the kinase as a result of the two hydroxyl groups on its B-ring, which consequently contributed to greater antioxidant activity over pinocembrin and naringenin.