Browsing by Subject "Listeria monocytogenes 10403S"
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    Antibacterial activity of Chrysanthemum indicum, Centella asiatica and Andrographis paniculata on Bacillus cereus and Listeria monocytogenes under Low pH Stress
    ( 2012) Patchanee Yasurin ; Nateepat Pitinidhipat ; Christina Vania Utami
    Classic food preservation methods do not guarantee to inhibit Bacillus cereus and Listeria monocytogenes growth. The application of natural antibacterial agent is an alternative way to control their growth. Asia herbs, Chrysanthemum indicum, Centella asiatica, and Andrographis paniculata were used in this experiment to test their antibacterial activity under low pH stress. Their antibacterial activities of 95% ethanolic crude extracts were tested on B. cereus and L. monocytogenes 10403S under different low pH stress by agar disc diffusion method. The best antibacterial effect on both bacteria was found at low pH stress condition. Lowering pH also acts as one inhibitory effect. The result of in vitro antibacterial effect as inhibition zone at pH 7.0, 6.5, 6.0, 5.5, showed that the inhibition zone diameters of C. indicum extracts were 7.62±1.18. 7.87±2.35, 6.25±3.06 and 9.50±2.14 cm, while the inhibition zone diameters of C. asiatica extracts were 8.75±1.03, 8.75±2.66, 7.75±2.37 and 9.12±1.96 cm and A. paniculata extracts were 9.75±1.75, 5.87±3.52, 8.5±1.23 and 9.33±1.63 against L. monocytogenes 10403S, respectively. Under the same condition, the inhibition zone diameters of C. indicum extracts at 2.12±0.64, 1.37±0.92, 0.93±0.78 and 6.00±3.25 cm, the inhibition zone diameters of C. asiatica extracts at 0.62±0.44, 2.25±0.46, 1.75±0.28 and 6.50±1.60 cm and A. paniculata extracts at 0.87±0.79, 1.25±0.60, 2.00±1.65 and 6.00±1.31, respectively against B. cereus. All 95% ethanolic crude extracts showed more inhibition effect on L. monocytogenes 10403S than B.cereus. However, the promising active antibacterial compounds in all three herbs are needed to be identified. The MBCs of A. paniculata, C. asiatica and C. indicum showed 4, 16 and >32 µl/ml against B. cereus while A. paniculata and C. asiatica showed 16 and >32 µl/ml against L. monocytogenes 10403S.
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    The fresh Thai local herbs in vitro antibacterial activity against Listeria monocytogenes 10403S
    (School of Biotechnology, Assumption University of Thailand, 2014-07) Patchanee Yasurin
    Thai local herbs are one important ingredient of Thai food. Six fresh herbs extracted by five different ethanol concentrations (0%, 25%, 50%, 75%, and 95%) were investigated for their individual antibacterial activity; Chilli (Capsicum annuum), kaffir lime (Citrus hystrix), cumin (Cuminum cyminum L.), shallot (Allium ascalonicum L.), garlic (Allium sativum), lemongrass (Cymbopogon citratus), and galangal (Alpinia galanga) against Listeria monocytogenes 10403S by using the agar disk diffusion method on Brain Heart Infusion media (BHI), five different ethanol concentrations (0%, 25%, 50%, 75%, and 95%). The results showed that 95 % ethanolic crude extracts gave the highest antibacterial activity against L. monocytogenes 10403S. Of all herbs, lemongrass extracted by 95% ethanol showed the highest antibacterial activity; 9.79 ± 1.68 mm. The minimum inhibitory concentrations (MICs), using a broth dilution method, were found to be between 16-128 µl/ml. The minimum bactericidal concentrations (MBCs), using a broth dilution method, were greater than 32-128 µl/ml. This indicated that all Thai local herbs showed the promising antibacterial activity against L. monocytogenes 10403S.
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    In-vitro Individual Antibacterial Activity Of Thai Red Curry Paste Ingredients Against Salmonella Enterica Enteritidis (Human) And Listeria Monocytogenes 10403s
    ( 2015) Patchanee Yasurin ; Treuktongjai Saenghiruna
    Thai red curry paste consists of herbs which have potential to be natural antibacterial agents. Therefore 7 herbs of Thai curry paste ingredients; Chili (Capsicum annuum), Kaffir lime (Citrus hystrix), Cumin (Cuminum cyminum L.), Shallot (Allium ascalonicum L.),Garlic (Allium sativum), Lemongrass (Cymbopogon citrates), and Galangal (Alpinia galangal), were investigated for their individual antibacterial activity againt Salmonella enterica Enteritidis (human) and Listeria monocytogenes 10403S by agar diffusion method Agar under 3 extraction3; water, UHT coconut milk and fresh coconut milk. In-vitro antibacterial screening results, fresh coconut milk extraction showed the highest potential in inhibiting S. enterica Enteritidis (human), in all herbs and the highest antibacterial activity was found in garlic; 01.00 ± 0.18 cm, using water extraction. For L. monocytogenes 10403S, the highest antibacterial activity was found in lemon grass using fresh coconut milk extraction; 0.90 ± 0.12 cm. The minimum inhibitory concentration (MICs), using broth dilution method, of all herbs were between 80 to >160µl/ ml. The minimum bactericidal concentrations (MBCs) of all herbs under three extractions, using broth dilution method, showed >160µl/ml in both bacteria. The herbs in Thai red curry paste ingredient showed the significantly promising antibacterial activity against food-borne pathogen, S. enterica Enteritidis (human) and L. monocytogenes10403S.
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    The individual antibacterial activity of Thai red curry paste’s dry ingredients against Listeria monocytogenes using different ethanolic extraction
    (School of Biotechnology, Assumption University of Thailand, 2014-07) Patchanee Yasurin
    Thai red curry paste is a main ingredient of Thai Red curry. The paste contains herbs which have the potential to be natural antibiotics. Six herbs contained in Thai red curry paste were tested for their individual antibacterial activity against Listeria monocytogenes 10403S. Six herbs tested were Chilli (Capsicum annuum), Kaffir lime (Citrus hystrixShallot (Allium ascalonicum L.), Garlic (Allium sativum), Lemongrass (Cymbopogon citratus), and Galangal (Alpinia galanga). The agar disk diffusion method was used for in-vitro screening antibacterial activity of each crude extract against L. monocytogenes 10403S on Beef Heart Infusion media (BHI) with five different ethanol concentrations; 0, 25, 50, 75, and 95%. The 95 % ethanol crude extracts gave the highest antibacterial activity against L. monocytogenes 10403S in all herbs. The dry galangal 95% crude ethanolic extract showed the highest antibacterial activity; 10.50 ± 1.16 mm. The minimum inhibitory concentrations (MICs), using a broth dilution method, were found to be between 4-128 µl/ml. The minimum bactericidal concentrations (MBCs), using a broth dilution method, were greater than 32-128 µl/ml. The results showed that the six dry herbs have significant antibacterial activity against the food-borne pathogen, L. monocytogenes 10403S.
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    Natural antibacterial activity of Thai curry paste in Thai red curry (Kang-Kati) model against Listeria monocytogenes 10403S
    (Rajamangala University of Technology Phra Nakhon, 2013) Patchanee Yasurin ; Thidarat Udchachonand
    In 2012, outbreak-associated strains of Listeria monocytogenes have been reported to CDC. Foods as natural antibiotics itself might be considered as an alternative for food safety. Thai red curry is a Thai cultural dish which has become well-known worldwide for having fresh coconut milk and red curry paste as main ingredients. The main ingredients of red curry paste include various types of herbs: Capsicum annuum (chili), Citrus hystrix (Kaffir lime), Cuminum cyminum L. (Cumin), Allium ascalonicum L. (Shallot), Allium sativum (Garlic), Cymbopogon citratus (Lemongrass), and Alpinia galangal (Galangal). All of the mentioned herbs have been investigated for their antibiotics activity. This project aimed to investigate the antibacterial activity of red curry paste in fresh-coconut-milk-based Thai red curry (Kang-Kati) model on L. monocytogenes 10403S. Thai curry paste in-vitro antibacterial activity was evaluated by standard plate count method on BHI media every hour for 6 hours at room temperature. Thai red curry was prepared by Thai homemade authentic cooking method as served in Thai cuisine. Results showed that the L. monocytogenes 10403S log CFU/ml level in Thai red curry (Kang-Kati) was significantly lower than in nutrient broth, as positive control, (P < 0.05) since 2nd - 6th hour; 2ndhr; 6.28±0.01 and 6.38±0.02, 3rdhr; 6.39±0.04 and 6.91±0.14, 4th; 6.96±0.05 and 7.24±0.10, 5th hr; 7.02±0.05 and 7.38±0.10 and 6th hr; 7.04±0.04 and 7.43±0.03 log CFU/ml, respectively. The t-test has been done by using SAS on log CFU/ml with P < 0.05. The Thai red curry paste in Kang-Kati model showed promising antibacterial activity against food-borne pathogenic bacteria, L. monocytogenes 10403S.
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    การศึกษาฤทธิ์ต้านจุลชีพของพริก แกงในแกงป่าในการยับยั้งเชื้อ Listeria monocytogenes 10403S
    (คณะอุตสาหกรรมเกษตร สถาบันเทคโนโลยีพระจอมเกล้าเจ้าคุณทหารลาดกระบัง, 2555-09-07) พัชนีย์ ยะสุรินทร์
    สารสกัดธรรมชาติที่มีฤทธิ์ต้านจุลชีพนั้นได้รับความสนใจอย่างมากโดยเฉพาะในด้านความปลอดภัยของอาหาร การศึกษาอาหารที่มีฤทธิ์ต้านจุลชีพนั้นเป็นเรื่องที่น่าสนใจ พริกแกงแดงเป็นเครื่องปรุงหลักที่ใช้ในการทําแกงป่า โดยทั่วไป ส่วนผสมของพริกแกงแดงประกอบไปด้วย พริกแห้ง กระเทียม หอมแดง ตะไคร้ ผิวมะกรูด ข่า และเมล็ดยี่หร่า งานวิจัยนี้ มีจุดประสงค์ที่จะศึกษาฤทธิ์ความเป็นไปได้ที่พริกแกงในแกงป่าจะมีฤทธิ์ในการยับยั้งเชื้อ Listeria monocytogenes 10403S แกงป่าเตรียมโดยใช้วิธีการต้ม1 ชั่วโมง ในสัดส่วน น้ํา 500 มิลลิลิตรต่อพริกแกง 45 กรัมรอให้เย็น และใส่เชื้อลงไป 1 % v/v ศึกษาฤทธิ์ต้านจุลชีพของพริกแกงโดยใช้วิธี in-vitro by cell count serial dilution ลงบนอาหารเลี้ยงเชื้อ BHI ทุกๆ 1 ชั่วโมงเป็นเวลา 6 ชั่วโมง ที่อุณหภูมิห้อง และควบคุมการปนเปื้อนจากเชื้อชนิดอื่นโดยการเก็บตัวอย่างจากชั่วโมงที่ศูนย์ ก่อนการถ่ายเชื้อ ผลการศึกษาแสดงให้เห็นว่าการเจริญเติบโตของ L. monocytogenes 10403S ในแกงป่านั้นต่ํากว่าอย่าง มีนัยสําคัญทางสถิติเมื่อเทียบกับ positive control (BHI) (P < 0.05) ตั้งแตชั่วโมงที่ 1-6, พริกแกงในแกงป่านั้นมีฤทธิ์ต้าน จุลชีพในการยับยั้งเชื้อ L. monocytogenes 10403S