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Purification and identification of bacterial cellulase activity of Bacillus subtilis W48 for biofuel production

by Patchanee Yasurin

Title:

Purification and identification of bacterial cellulase activity of Bacillus subtilis W48 for biofuel production

Author(s):

Patchanee Yasurin

Issued date:

2014-07

Publisher:

School of Biotechnology, Assumption University of Thailand

Citation:

International Conference on Sustainable Global Agricultural and Food Security

Abstract:

Lignocellulosic biomass is a renewable, inexpensive, and abundant resource with high potential for biofuel production to implement the sustainable energy worldwide. An important key of biofuel production is the hydrolysis of lignocellulosic biomass to fermentable sugars. Screening for a novel cellulase, as a biocatalyst, is challenge for development of biofuel production to be the economic and environmental friendly process. Natural environment is an important source to find an efficient cellulase producing bacteria. Previously, Bacillus subtilis strain W48 was screened and isolated from rainforest park at Assumption University, Bangkok, Thailand. In this study, we focused on the purification and characterization of cellulase enzyme produced by B. subtilis strain W48. The crude cellulase enzyme was collected by culture grown at 45°C in carboxy-methyl-cellulose (CMC) media. Then, it is concentrated by ammonium sulfate precipitation and fractionated by using size exclusion chromatography using sephacryl S- 100 HR column. In this study, the fractions that has highest CMCase activity (0.017 mg/ml), as endoglucanase, and FPase activity (0.013 mg/ml), exoglucanase, are fraction no. 13 and 11, respectively.

Keyword(s):

Bacillus subtilis W48
Cellulase
Purification

Resource type:

Conference Paper

Extent:

4 pages

Type:

Text

File type:

application/pdf

Language:

eng

URI:

http://repository.au.edu/handle/6623004553/17763
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Copyright © Assumption University.
All Rights Reserved.

Contact Us

The St. Gabriel's Library   
Hua Mak Campus  
Ramkhamhaeng 24, Hua Mak  
Bangkok Thailand 10240  
Tel.: (662) 3004543-62 Ext. 3402  
Fax.: (662) 7191544  
E-Mail Library : library@au.edu  


The Cathedral of Learning Library
Suvarnabhumi Campus
Bang Na-Trad Km. 26 Bangsaothong
Samuthprakarn Thailand 10540
Tel.: (662) 7232024, 7232025
Fax.: (662) 7191544
E-Mail Library : library@au.edu
 

 

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