Effect of ohmic pretreatment on tissue integrity and extraction yield of Chinese chives (Allium tuberosum) leaf oil

au.link.externalLink [Full Text] (https://www.researchgate.net/publication/305221340_Effect_of_ohmic_pretreatment_on_tissue_integrity_and_extraction_yield_of_Chinese_chives_Allium_tuberosum_leaf_oil)
dc.contributor.author Patchanee Yasurin
dc.date.accessioned 2016-03-17T07:46:29Z
dc.date.available 2016-03-17T07:46:29Z
dc.date.issued 2015-11
dc.description.abstract Chinese chives (Allium tuberosum Rottl.) are known for its antimicrobial and antioxidant properties. However, extract from Chinese chives was not commercially available due to very low extraction yield of the conventional method, steam distillation. This study focused on effect of ohmic pretreatment at different electric field strengths on tissue integrity and extraction yield of Chinese chives leaf oil. The field strength was varied at E = 0, 25, 50, 75 and 100 V/cm. For all treatment, the cut-off temperature was fixed at 60 °C. The tissue integrity was determined by an electrical conductivity disintegration index (Z) and viable staining using neutral red dye. It was found that as the field strength increased, Z value increased indicating a higher degree of tissue damage. The highest degree of tissue damage (Z ~ 0.8) was obtained when E > 75 V/cm were applied. The viability test showed agreeable results to the Z values. More red cells were observed at the lower field strength. At E = 25 v/cm, the amount of viable cells were comparable to the control (fresh tissue) while there was almost no viable cell left at E > 75 V/cm. When ohmic pretreatment at E = 75 V/cm was applied prior to 2.5 h of steam distillation, the extraction yield was increased from 0.12+0.01% to 0.16+0.02% by fresh leaf weight or about 33%. Thus, ohmic pretreatment was suggested to increase the permeability of any biological materials prior to extraction process in order to enhance the extraction yield. en_US
dc.format.extent 11 pages en_US
dc.format.mimetype application/pdf en_US
dc.identifier.citation The 27th Annual Meeting of the Thai Society for Biotechnology and International Conference en_US
dc.identifier.uri https://repository.au.edu/handle/6623004553/17773
dc.language.iso eng en_US
dc.subject Chinese chives en_US
dc.subject Extraction yield en_US
dc.subject Ohmic en_US
dc.subject Tissue integrity en_US
dc.subject Viability staining en_US
dc.title Effect of ohmic pretreatment on tissue integrity and extraction yield of Chinese chives (Allium tuberosum) leaf oil en_US
dc.type Text en_US
mods.genre Conference Paper en_US
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