The Development of Centella asiatica Extract-Loaded BSA Nanoparticles Production to Improve Bioavailability

au.identifier.callno P294d
dc.contributor.author Patchanee Yasurin
dc.date.accessioned 2017-11-08T02:26:14Z
dc.date.available 2017-11-08T02:26:14Z
dc.description Includes bibliography.
dc.description.abstract Centella asiatica cBao-bog) is used as a traditional drug widely in Asia. C. asiatica crude extracts showed excellent potential in-vitro but less or no in-vivo activity due to their poor lipid solubility or improper molecular size or both, resulting in poor absorption, poor dosing and poor bioavailability. The Bovine serum albumin cBSA) can attract macromolecular and carry wide variety of molecule. So this research was aimed to develop C. asiatica extract-loaded BSA nanoparticles cCBNP) to improve bioavailability. CBNP was prepared by the desolvation method using three different ratio C. asiatica crude chloroform extracts: BSA cl:2, 1:3, and 1:4). The well agar diffusion method was used for evaluating antibacterial activity of CBNP with different concentration (100, 200, and 300 µg!Illl) against five food borne pathogens cEscherichia coli ATCC25822, Salmonella enterica Typhimurium U302 cDT104b), S. enterica Enteritidis chuman), S. enterica 4,5,12:i:- chumall) US clone, and Bacillus cereus). The results showed that the antibacterial activity of CBNP did not show significant different on three different ratio and concentration in all food borne pathogens except S. enterica Enteritidis chuman) and B. cereus cP < 0.05). The highest antibacterial active of CBNP was 1.07±0.46 cm against S. enterica Enteritidis (human) on ratio 1 :4, 200 µg!Illl. The antibacterial activity of CBNP gave almost 2 times highe than free crude C. asiatica chloroform extracts. The modified Folin-Ciocalteu method, Ferric reducing antioxidant potential assay and DPPH assay were used for evaluating antioxidant activity. The highest antioxidant activity represented by the amount of phenolic content of CBNP was 14.59±6.74 µgGAEIIllg using ratio 1:2. The amount of phenolic content of CBNP did not show significant different between ratio 1 :2 and others cP < 0.05). For Ferric reducing antioxidant potential assay and DPPH assay, crude chloroform extract showed significantly higher activity than CBNP were 1.00 ± 0.15 mmol Fe2 + 1Illg and 29.44 ± 8.20 % DPPH radical scavenging respectively cP < 0.05). The entrapment efficiency and loading efficiency of CBNP showed highest value on ratio 1 :4 which were 96.94 ± 1.48% and 42.05 ± 5.68% respectively. It's also showed highest in release kinetic In Vitro approximately 12% during the whole period of 6 hours in both artificial gastric and intestinal juice. C. asiatica crude chloroform extract have higher solubility in water than CBNP but CBNP have higher stability in releasing crude chloroform extract. The results indicated that CBNP showed the promising to increase bioavailability of C. asiatica. en_US
dc.format.extent 77 pages en_US
dc.format.mimetype application/pdf en_US
dc.identifier.uri https://repository.au.edu/handle/6623004553/20046
dc.language.iso eng en_US
dc.publisher Assumption University en_US
dc.rights This work is protected by copyright. Reproduction or distribution of the work in any format is prohibited without written permission of the copyright owner. en_US
dc.rights.holder Assumption University en_US
dc.subject Centella asiatica en_US
dc.subject Nanoparticles en_US
dc.subject Bioavailability en_US
dc.subject Antibacterial en_US
dc.subject Antioxidant en_US
dc.title The Development of Centella asiatica Extract-Loaded BSA Nanoparticles Production to Improve Bioavailability en_US
dc.type Text en_US
mods.genre Research Report en_US
mods.location.physicalLocation 4th Floor (Cathedral of Learning) en_US
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