Detection of hepatocellular carcinoma in transgenic mice by Gd-DTPA- and rhodamine 123-conjugated human serum albumin nanoparticles in T1 magnetic resonance imaging
Detection of hepatocellular carcinoma in transgenic mice by Gd-DTPA- and rhodamine 123-conjugated human serum albumin nanoparticles in T1 magnetic resonance imaging
dc.contributor.author | Waralee Watcharin | |
dc.contributor.author | Schmithals, Christian | |
dc.contributor.author | Pleli, Thomas | |
dc.contributor.author | Köberle, Verena | |
dc.contributor.author | Korkusuz, Hüdayi | |
dc.contributor.author | Hübner, Frank | |
dc.contributor.author | Waidmann, Oliver | |
dc.contributor.author | Zeuzem, Stefan | |
dc.contributor.author | Korf, Horst-Werner | |
dc.contributor.author | Terfort, Andreas | |
dc.contributor.author | Gelperina, Svetlana | |
dc.contributor.author | Vogl, Thomas J. | |
dc.contributor.author | Kreuter, Jörg | |
dc.contributor.author | Piiper, Albrecht | |
dc.date.accessioned | 2016-03-18T03:15:25Z | |
dc.date.available | 2016-03-18T03:15:25Z | |
dc.date.issued | 2015 | |
dc.description.abstract | Nanoparticle (NP)-based contrast agents that enable high resolution anatomic T1-weighted magnetic resonance imaging (MRI) offer the prospect of improving differential diagnosis of liver tumors such as hepatocellular carci- noma (HCC). In the present study, we investigated the possibility of employing novel non-toxic human serum albumin nanoparticles conjugated with Gd-DTPA and rhodamine 123 (Gd-Rho-HSA-NPs) for the detection of HCC by T1-weighted MRI. In addition, the influence of surface coating of the NPs with poloxamine 908, which al- ters the absorptive behavior of NPs and changes their distribution between the liver and tumor was examined. MRI of transgenic mice with endogenously formed HCCs following intravenous injection of Gd-Rho-HSA-NPs revealed a strong negative contrast of the tumors. Contrasting of the HCCs by NP-enhanced MRI required less Gd as compared to gadolinium-ethoxybenzyl-diethylenetriaminepentaacetic acid-enhanced MRI, which current- ly provides the most sensitive detection of HCC in patients. Immunohistochemical analyses revealed that the Gd-Rho-HSA-NPs were localized to macrophages, which were – similar to HCC in patients – fewer in number in HCC as compared to the liver tissue, which is in agreement with the negative contrasting of HCC in Gd-Rho- HSA-NP-enhanced MRI. Poloxamine-coated NPs showed lower accumulation in the tumor macrophages and caused a longer lasting enhancement of the MRI signal. These data indicate that Gd-Rho-HSA-NPs enable sensi- tive detection of HCC by T1-weighted MRI in mice with endogenous HCC through their uptake by macrophages. Poloxamine coating of the NPs delayed the tumor localization of the NPs. | en_US |
dc.format.extent | 9 pages | en_US |
dc.format.mimetype | application/pdf | en_US |
dc.identifier.citation | Journal of Controlled Release 199 (2015) 63–71 | en_US |
dc.identifier.uri | https://repository.au.edu/handle/6623004553/17781 | |
dc.language.iso | eng | en_US |
dc.subject | MRI | en_US |
dc.subject | HCC | en_US |
dc.subject | Macrophages | en_US |
dc.subject | Gadolinium | en_US |
dc.subject | Nanoparticle | en_US |
dc.subject | Targeting | en_US |
dc.title | Detection of hepatocellular carcinoma in transgenic mice by Gd-DTPA- and rhodamine 123-conjugated human serum albumin nanoparticles in T1 magnetic resonance imaging | en_US |
dc.type | Text | en_US |
mods.genre | Article | en_US |
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