Development of corn silk as a biocarrier for Zymomonas mobilis biofilms in ethanol production from rice straw
Development of corn silk as a biocarrier for Zymomonas mobilis biofilms in ethanol production from rice straw
Files (excerpt)
Published date
2016
Resource type
Publisher
ISBN
ISSN
DOI
Call no.
Other identifier(s)
Edition
Copyrighted date
Language
eng
File type
application/pdf
Extent
7 pages
Other title(s)
Advisor
Other Contributor(s)
Citation
The Journal of General and Applied Microbiology 62.2 (April 2016), 68-74
Degree name
Degree level
Degree discipline
Degree department
Degree grantor
Abstract
Z. mobilis cell immobilization has been proposed
as an effective means of improving ethanol production.
In this work, polystyrene and corn silk were
used as biofilm developmental matrices for Z.
mobilis ethanol production with rice straw hydrolysate
as a substrate. Rice straw was hydrolyzed
by dilute sulfuric acid (H2SO4) and enzymatic hydrolysis.
The final hydrolysate contained furfural
(271.95 ± 76.30 ppm), 5-hydroxymethyl furfural
(0.07 ± 0.00 ppm), vanillin (1.81 ± 0.00 ppm),
syringaldehyde (5.07 ± 0.83 ppm), 4-
hydroxybenzaldehyde (4-HB) (2.39 ± 1.20 ppm) and
acetic acid (0.26 ± 0.08%). Bacterial attachment or
biofilm formation of Z. mobilis strain TISTR 551
on polystyrene and delignified corn silk carrier
provided significant ethanol yields. Results showed
up to 0.40 ± 0.15 g ethanol produced/g glucose consumed
when Z. mobilis was immobilized on a polystyrene
carrier and 0.51 ± 0.13 g ethanol produced/
g glucose consumed when immobilized on
delignified corn silk carrier under batch fermentation
by Z. mobilis TISTR 551 biofilm. The higher
ethanol yield from immobilized, rather than free
living, Z. mobilis could possibly be explained by a
higher cell density, better control of anaerobic conditions
and higher toxic tolerance of Z. mobilis
biofilms over free cells.